More than 300 suspects have been eliminated from the investigation into the brutal murder of a nurse found gagged, handcuffed and beaten to death nearly 23 years ago.

Mother-of-three Janet Brown was found dead in her home in Sprigs Holly Lane in Radnage on April 11, 1995, with the devastating discovery sending shockwaves through the village.

Her killer has never been found and brought to justice.

Exactly 20 years on, in April 2015, a fresh appeal for information was launched by Thames Valley Police detectives, with renewed hope of catching the killer thanks to the discovery of a new sample of DNA.

It has now been revealed by police that 300 potential suspects have been ruled out by having their DNA examined. No arrests have been made since the renewed appeal was launched.

Mrs Brown, who worked at Oxford University, had been alone in the family’s home on the evening of the murder.

Her husband, Graham, was away in Switzerland working at the time, while two of her children no longer lived at the property and her youngest daughter, Roxanne, was at a friend’s house.

It is believed the attack happened between 8pm and 10pm, before her naked body was discovered by a builder and his teenage son the following day.

Mrs Brown had been struck to the head a number of times with what police described as a heavy implement, possibly a metal bar.

Peter Beirne, from the major crime review unit, said: “We would continue to appeal to anyone with any information about Janet’s murder to contact police on 101.”